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KMID : 0380620190510010024
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 1 p.24 ~ p.28
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In vitro hepatocyte inflammation by Ephedra sinica extracts
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Kim Il-Rang
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Abstract
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In this study, the in vitro hepatotoxic mechanism of Ephedra sinica (ma-huang) was investigated by measuring the degree of cell death, secretion of cytokine, and fat accumulation by treating HepG2 cells with 70% ethanolic extracts of ma-huang. Cell death was observed at concentrations of around 5-100 μg/mL by treatment with ma-huang extracts (p<0.05). The secretion of interleukin 8 (IL-8) and macrophage colony-stimulating factor (M-CSF), which are inflammatory cytokines, were significantly promoted at concentrations of around 0.05-100 and 0.5-100 μg/mL, respectively (p<0.05). In this experiment, it was shown that the extracts of ma-huang stimulate the secretion of inflammatory cytokines, such as IL-8 and M-CSF, and lead to fat accumulation in the hepatocytes, thereby causing inflammation of the hepatocytes. Hepatotoxicity was observed at around 10-500 times lower concentration than the concentration required to cause serious toxicity, such as cell death, suggesting that hepatic toxicity (hepatitis) may be induced at a low dose.
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KEYWORD
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Ephedra sinica (ma-huang), inflammation, interleukin-8, macrophage colony-stimulating factor, lipid accumulation
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